Disinfectant virus killing experiment

Disinfectant virus killing experiment

experimental background

Disinfection is the use of physical, chemical and biological methods to kill pathogenic microorganisms, the purpose of which is to prevent and control the occurrence and spread of infectious diseases. Whether these hundreds of disinfectants really have virus-killing effects also requires professional third-party testing agencies to test and verify the virus-killing effects of disinfectants.

test subject

Disinfectants (including aerosol disinfection, smear disinfection, soaking disinfection and other products)

test standard

"Disinfection Technical Specifications" (2002 Edition)
Group standard "Test method for inactivation of hand, foot and mouth disease virus by disinfectant"
Group Standard "Test Method for the Effect of Disinfectants on Inactivating Adenovirus"
National standard "Test method for the inactivation effect of disinfectants on respiratory viruses"

trial service

You can provide your own test plan or entrust our company to formulate a test plan according to the characteristics of the tested product.
The result evaluation of disinfectant products includes neutralizing agent identification, and a neutralizing agent identification fee needs to be charged.

Type of virus test

Herpes simplex virus (ATCCVR-1493)
Herpes zoster virus (ATCCVR-1433)
Coronavirus 229E (ATCCVR-740)
Influenza virus H1N1 (ATCCVR-1469)
Influenza virus H3N2 (ATCCVR-544)
Adenovirus type 5 (ATCCVR-5)
Enterovirus EV71 (ATCCVR-1432)
Coxsackievirus A18 (ATCCVR-176)
Rotavirus (ATCCVR-2018)

test flow

1. Appropriately dilute the HFMD virus suspension and inoculate it into Vero cells.
2. Add an appropriate amount of cell maintenance medium, place it in a 37 ° C, 5% carbon dioxide cell incubator, and observe CPE daily, usually for 3 to 5 days.
3. When it is found that the cells start to fall off in large groups from the bottom of the culture bottle, and 75% of the cells appear CPE, immediately freeze and thaw the cells three times (alternately at -20 ° C and 37 ° C), suck the virus suspension into the centrifuge tube, and centrifuge for 6000r/min for 10min to remove the precipitation.
4. After centrifugation, take the supernatant, put it in a cryopreservation tube, and store it in a refrigerator at -80 ° C.
5. Calculation of viral infection titer by endpoint dilution method.
6. Evaluation of disinfection effect: According to the use concentration (intensity) and action time specified in the product instruction manual, the test is repeated 3 times. The inactivation log value of each test is ≥ 4.00, which can determine that the inactivation effect of the product on hand, foot and mouth disease virus meets the disinfection qualification requirements; the positive control group virus titer log value should not be less than 6.00.