Bacterial detection

Bacterial detection

 

Routine testing items:Strain identification, molecular identification, vitality identification, safety identification, biochemical identification, etc
 

identification method	Involve species
rDNA (ribosomal DNA) sequence analysis	Bacteria, Actinomycetes, Yeast, Mold
	regular filamentous fungus
Functional gene analysis	Lactobacillus, Bifidobacteria, Enterococcus
（pheS,gyrB,gyrA,atpD等）	Bacillus
Physiological and biochemical analysis	Bacteria, Actinomycetes, Yeast
BioLog Analysis	Bacteria, yeast
API	Bacteria, Saccharomyces cerevisiae
Morphological analysis	mold
1, 2 are combined with 3, 4, and 5 respectively	Bacteria, Actinomycetes, Yeast, Mold
	regular filamentous fungus
Determination of G + C mol% content	Bacteria, Actinomycetes, Yeast
Fatty acid composition analysis	Bacteria, Actinomycetes, Yeast
DNA-DNA hybridization	Bacteria, Actinomycetes, Yeast
polar lipid	Bacteria, Actinomycetes
Respiratory quinone	Bacteria, Actinomycetes
electron microscope	Bacteria, Actinomycetes, Yeast, Mold

 

Iron bacterial assay

Test standard reference

ASTM D932-2015 Test Method for Iron Bacteria in Water and Water Sediments

GB/T 14643.6-2009 Determination of bacteria and algae in industrial circulating cooling water, Part 6: Determination of iron bacteria MPN method

HY/T 176-2014 Determination of iron bacteria in seawater by MPN method

JIS K0350-80-10-2005 Test method for the detection of iron bacteria in industrial water

SY/T 0532-2012 Oilfield injection water bacterial analysis method, extinction dilution method

 

Bacterial activity testing, enzyme activity testing, etc

Test standard reference

BS EN 14349-2007 Chemical disinfectants and preservatives - Quantitative surface tests for the evaluation of bacterial activity of chemical disinfectants and preservatives for non-mechanically moving non-porous surfaces in the veterinary field - Test methods and requirements (phase 2/step 2)

GB/T 38483-2020 Determination of antibacterial activity of secondary metabolites of microbial antibiotics, bacteriostatic circle method

QB/T 5482-2020 Chemical disinfectants and fungicides, basic disinfectant activity, test methods and requirements

QB/T 5484-2020 Evaluation of bactericidal activity of disinfectants and fungicides for food and household use

 

Test item: Determination of bacterial growth curve

Testing fee: free initial inspection. After the initial inspection, the quotation will be made according to the customer's testing needs and the complexity of the experiment.

Bacterial growth curve refers to single-celled microorganisms. It is to inoculate a small amount of single-celled microorganisms into a certain volume of liquid medium, culture them under suitable conditions, and regularly sample and measure the number of cells. Take the logarithm of the number of cells growing as the vertical coordinate and the culture time as the horizontal coordinate to draw a curve. We call this curve the bacterial growth curve.

Determination steps

(1) Bacterial liquid culture: Pick a ring of fungus moss on the inclined surface culture of the strain, inoculate it in a thousand broth medium, and culture it at 3 TC for about 12 hours. This bacterial liquid is the seed culture liquid.

(2) Divide the medium and correct the zero point: Pipette 25 mL of medium with a sterile pipette and add it to a triangular flask with a side arm tube. Pour the uninoculated medium into its side arm tube and adjust the zero point on the photoelectric colorimeter. Even if the OD value on the photoelectric colorimeter is above zero.

(3) Inoculation and zero-hour determination: Pipette the 2. SmL seed culture solution into the inoculation bottle and shake it well. Pour the freshly inoculated culture solution into the side arm test tube and measure the OD value of the photoelectric colorimeter. The reading at this time is the zero-hour reading value in the growth curve of the strain after inoculation (ie, inoculation wipe).

(4) Cultivation and growth measurement The triangular flask measured at 0 o'clock is immediately placed on a constant temperature water bath shaker for culture. The culture temperature is 3 TC, and the frequency of the shaker is about 100 times/min (the temperature and frequency can be adjusted at any time). During the culture, the triangular flask should be removed from the shaker every 30 minutes, the bacterial liquid should be poured into the side arm test tube, and the OD value should be read on the photoelectric colorimeter. Record the measured data each time. During each measurement, use the culture solution of a blank control tube to correct the zero point of the photoelectric colorimeter.

(5) Drawing the growth curve: taking the measured time as the abscissa, the logarithm of the number of bacteria (OD value) as the ordinate, and drawing points on the semi-logarithmic coordinate paper, the resulting curve is the growth curve of the measured shade under experimental conditions.

 

Bacterial recovery mutation test

Inspection cycle: 7-10 working days

Testing fee: free initial inspection. After the initial inspection, the quotation will be made according to the customer's testing needs and the complexity of the experiment.

Report Type: Sales/R & D/Industrial Problem Diagnosis/Judicial/Research Paper Data Use

 

Bacterial recovery mutation test standard

GB 15193.4-2014 Food safety standard, bacterial recovery mutation test

GB/T 15670.14-2017 Toxicological test methods for pesticide registration, Part 14: Bacterial recovery mutation test

GB/T 21786-2008 Chemicals, bacterial recovery mutation test method

Genotoxicity test for YY/T 0870.1-2013 medical apparatus - Part 1: Bacterial recovery mutation test

 

Bacterial recovery mutation test

Little knowledge of bacterial recovery mutation test

"Chemicals: Test Methods for Bacterial Recovery Mutation (GB/T 21786-2008) " was proposed and centralized by the Standardization Technical Committee for the Management of Hazardous Chemicals (SAC/TC 251). "Chemicals: Test Methods for Bacterial Recovery Mutation (GB/T 21786-2008) " was responsible for the drafting unit: Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention. Participating drafting units: Beijing Center for Disease Control and Prevention, Ningbo Entry-Exit Inspection and Quarantine Bureau. "Chemicals: Test Methods for Bacterial Recovery Mutation (GB/T 21786-2008) " was issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China - China Standardization Administration Committee in May 2008 and implemented on September 1, 2008.

 

Other items: bacterial number detection, bacterial polysaccharide detection

 

Bacterial testing standards

AS 1766.1.4-1991 Microbiology of food, Part 1: Specific examination of living organisms, Section 1.4: Determination of bacterial number, Test method for classification of surface spread

AS 1766.1.6-1991 Microbiology of food, Part 1: Special examination of living organisms, Section 1.6: Method of counting bacteria using the technique of greater probability number

ASTM D932-2015 Test Method for Iron Bacteria in Water and Water Sediments

ASTM D4454-1985 (2009) Test Method for Simultaneous Counting of the Total Number of Respiratory Bacteria in Water by Microscopic Method

ASTM D4783-2001 (2013) Test Method for Erosion Resistance of Adhesive Articles in Containers by Bacteria, Yeast, and Fungi

ASTM E723-2007 Test Method for the Effectiveness of Antimicrobial Agents (Bacterial Spoilage) as Preservatives for Aqueous Products in the Paper Industry

ASTM E1326-2008 Guidelines for the Evaluation of Unconventional Microbial Tests for Bacterial Counting

ASTM E1839-2007 Test method for efficacy of myxicides for bacterial and fungal mucus in the paper industry

ASTM F838-2005 Test Method for Determination of Bacterial Retention in Membrane Filters for Liquid Filtration

ASTM F2101-2007 Test Method for Evaluating Bacterial Filtration Efficiency of Medical Mask Materials Using Staphylococcus aureus Biosensors