Extraction and extraction of volatile components to improve double
Abstract: Using sub-boiling water extraction, flocculation and alcohol precipitation to achieve the extraction and purification of Shuanghuanglian, to achieve the purpose of reducing the dose. The best solution is as follows: 100g of medicinal materials after supercritical extraction, add 10 times the volume of water, cook at 90 ° C for 2h, filter, the filtrate is concentrated to 80mL, at 50 ° C, add 1.5% flocculant 133mL, stir for 5min, centrifuge, concentrate the supernatant to 16mL, add 95% ethanol to 80% alcohol content, filter, and the filtrate is evaporated to dry, and the yield is about 5-6%.
Shuanghuanglian preparations (including Shuanghuanglian granules) are based on the selection of many ancient recipes and the removal of coarse and refinedBaicalin), Forsythia composition of this prescription, after years of clinical research proved that it has a good antibacterial, antiviral, anti-inflammatory antipyretic function, clinically widely used as upper respiratory tract infections [1], tonsillitis, pneumonia [2] and other preferred drugs. Shuanghuanglian preparation has a long history of use, as early as the early 1970s has been used in traditional Chinese medicine clinical for viral infections. Long-term clinical application has proved that it is a good efficacy, clear main symptoms of the prescription, in the early 1990s, its series of preparations, including powder injection, water injection, oral liquid, aerosol, tablets, etc. However, there are still some shortcomings in the above dosage forms, such as: water injection has the disadvantage of poor stability [3]; powder injection has considerable difficulty in production due to high technical requirements and long production cycle [4]; these two injections are prone to infusion reactions [5, 6], so their use is limited. In addition to unstable factors, oral liquid also has disadvantages such as high cost and inconvenience to carry. At the same time, various preparations do not have controllable Quality Standards in line with international standards. This study uses a combination of flocculation and alcohol precipitation to refine Shuanglonglian, hoping to improve the efficacy and reduce the dose.
HoneysuckleForsythia forsythia contains a large amount of volatile oil, which has good curative effect against viruses, but the old process is direct water extraction, resulting in the loss of a large amount of volatile components. In order to improve the therapeutic effect of Shuanghuanglian extract, we first use supercritical extraction to extract volatile components. Using a uniform design method, the active ingredients of Shuanghuanglian can be better extracted by sub-boiling water extraction. Using orthogonal experiments, the purification of Shuanghuanglian is realized by a combination of flocculation and alcohol precipitation. In the final preparation process, volatile components are added to ensure its curative effect. In the flocculation treatment, the addition of flocculants can remove most of the protein, pectin, tannin and other colloidal components in the liquid through adsorption bridging and electric neutralization, while the polysaccharides and chlorogenic acid and other components are better retained. Through alcohol precipitation treatment, the active ingredients in the liquid such as chlorogenic acid are soluble in both alcohol and water, and the insoluble components in the liquid, such as polysaccharides and remaining chitosan flocculants, can be precipitated in the aqueous alcohol solution to remove the insoluble components in the liquid, such as polysaccharides and remaining chitosan flocculants, etc., to further purify the liquid, greatly reduce the product yield, and increase the content of the active ingredient chlorogenic acid. The obtained extract has been verified by further animal experiments, and good results have also been obtained, ensuring the curative effect and reducing the dosage, so that the improvement of the dosage form from oral liquid to capsule can be realized.
References:
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