A-Plus™ Poly(A) Polymerase Tailing Kit

2026-03-27 10:24:45

A-Plus™ Poly(A) Polymerase Tailing Kit

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brand	CELLSCRIPT	item number	C-PAP5104H
Product classification	RNA Transcription Kit	Research Area
Product Overview	Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C
form	The A-Plus™ Poly(A) Polymerase Tailing Kit uses ATP as a substrate for template-independent additionof adenosine monophosphates to the 3'-hydroxyl termini of RNA. The standard prot°Col produces apoly(A)-tail length of ~150 b on 40-60 μg of RNA.Polyadenylation increases the stability of RNA ineukaryotic cells and enhances its ability to be translated after transfection or microinjection.1-3 A Poly(A)tail is useful to provide a priming site for first-strand cDNA synthesis in certain applications, and can beused to end-label4 or quantify5 mRNA.
Product description	Unit Definition One unit of A-Plus Poly(A) Polymerase converts 1 nmole of ATP into acid-insoluble material in 10minutes at 37°C under standard assay reaction conditions. Functional Testing The A-Plus Poly(A) Polymerase Tailing Kit is functionally tested in 1X A-Plus Poly(A) Tailing Bufferwith 1 mM ATP and a 1.4 kb transcript. Contaminating Activity Assays All components of the A-Plus Poly(A) Polymerase Tailing Kit are free of detectable RNase and DNaseactivity.
official website	http://www.cellscript.com/products/002pl0115CS.pdf
quantity	nclick="reduce_pay(176574);"> nclick="add_pay(176574);">

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