Cap-Clip™ Acid Pyrophosphatase
Cap-Clip™ Acid Pyrophosphatase
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CELLSCRIPT |
item number |
C-CC15011H |
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Product classification |
RNA Transcription Kit |
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Product Overview |
Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C
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Cap-Clip™ Acid Pyrophosphatase was developed as an improvement over Tobacco AcidPyrophosphatase (TAP). Cap-Clip Acid Pyrophosphatase can be used unit for unit in any previouslydeveloped prot°Col that utilized TAP. The Reactions Buffers for the two enzymes are identical. Cap-Clip Acid Pyrophosphatase is a plant-derived enzyme that hydrolyzes various pyrophosphate bonds,including the pyrophosphate bonds of the 5'-terminal m7GpppG "cap" of eukaryotic messenger RNAs, aswell as 5' cap structures on many small nuclear RNAs (snRNAs), heterogeneous nuclear RNAs and someviral RNAs. Complete hydrolysis of such capped RNAs generates RNA that has a 5'-monophosphategroup. With lot to lot consistency, absence of critical contaminants and a dependable inventory, Cap-Clip AcidPyrophosphatase replaces all TAP needs.
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Product description |
Unit Definition One unit of Cap-Clip Acid Pyrophosphatase releases one nanomole of inorganic phosphate fromm7GpppG in 30 minutes at 37°C under standard assay conditions. Contaminating Activity Assays Cap-Clip Acid Pyrophosphatase is free of detectable RNase, DNase and phosphatase activities
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