Intestinal bacteria increasing broth (EE)
[Number]: VIP (HB) 0126
[Product name]: Intestinal bacteria increasing broth (EE)
[Specifications]: 250g
[Purpose]:
[Product name]: Intestinal bacteria increasing broth (EE)
[Specifications]: 250g
[Purpose]:
Intestinal bacteria increasing broth (EE)
Name: Enterobacteria Enrichment Broth
Part Number: VIP (HB) 0126
Specifications: 250g
Purpose: used forIntestinal bacteriaBacterial culture (GB standard)
Intestinal bacteria increasing brothComposition (g/L)
peptone 10.0
Glucose 5.0
Disodium hydrogen phosphate 8.0
Potassium dihydrogen phosphate 2.0
Bovine Bile Salt 20.0
Yellow green 0.015
pH 7.2 ± 0.2 25 ℃
Intestinal bacteria increasing brothusage
Weigh 45 grams of this product, heat it and stir it to dissolve in 1000ml of distilled water, divide it into triangular bottles, each bottle is 90ml, heat it at 100 ° C for 30 minutes, and quickly cool it in flowing cold water without autoclaving.
Quality control and typical characteristics
After the preparation of this medium is a green liquid, inoculate 10-100 E. coli at 36 ± 1 ℃ for 18-24h, should grow well.
The growth characteristics of bacteria in the broth:
Staphylococcus aureus was inhibited on EE broth without discoloration. Escherichia coli, Enterobacter cloacae, Salmonella typhimurium were turbid and yellow.
Microbial sensitivity test of intestinal bacteria-enriched broth (EE):
Prepare the medium according to the label usage, inoculate the following quality control strains, and place them at 36 ± 1 ° C for aerobic culture for 18-24 hours
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
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