Sorbitol McConkay Agar Base (SM

2026-03-27 10:24:45

  [Number]: VIP (HB) 0121

  [Product Name]: Sorbitol McConkay Agar Base (SMAC)

  [Specifications]: 250g

  [Purpose]:

　　Sorbitol McConkay Agar Base (SMAC) (SN Standard)

　　Name: Sorbitol MacOSAgar Bunder

　　Part Number: VIP (HB) 0121

　　Specifications: 250g

　　Purpose: It is used for selective isolation and culture of Escherichia coli O157.

　　Additives: 5 boxes of VIP (HB) 0121-1 need to be added to each box. Sorbitol McConkey AgarAdditives are used.

Sorbitol McConkay Agar BaseComposition (g/L)

　　Peptone 17.0g

　　Polyvalent peptone 3.0g

　　Sorbitol 10.0g

　　Bovine bile salt 5.0g

　　Sodium chloride 5.0g

　　Neutral red 0.025g

　　Crystal violet 0.001g

　　Agar 17g

　　PH value 7.2 ± 0.2 25 ℃

Sorbitol McConkay Agar Baseusage

　　Weigh 51.5 grams of this product, dissolve it in 1000ml of distilled water, divide it into triangular bottles, 200ml each, autoclave at 121 ° C for 20 minutes, cool it to 45-50 ° C and add 0.5mg of sterile potassium tellurite and 0.01mg of thiosporin (or add a piece of sorbitol McConkay agar additive), mix well, pour into a sterile plate, set aside.
山梨醇麦康凯琼脂基础

　　Basic microbial sensitivity test of sorbitol McConkay agar:

　　Inoculate 10-100 CFU or less and incubate at 36 ± 1 ℃ for 18-24 hours.

　　Note: When calculating the collection rate, TSA agar was used as the control medium.
山梨醇麦康凯琼脂基础

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