Modified EC Broth (mEC + n)
[Number]: VIP (HB) 0147-1
[Product Name]: Modified EC Broth (mEC + n)
[Specifications]: 250g
[Purpose]:
[Product Name]: Modified EC Broth (mEC + n)
[Specifications]: 250g
[Purpose]:
Modified EC Broth (mEC + n)
Name: Modified EC Broth
Part Number: VIP (HB) 0147-1
Specifications: 250g
Usage: For 0157 selective enrichment (GB standard)
Additives: 6 boxes of VIP (HB) 0106-1 need to be added to each bottle. neomycin4.5 mg is used.
Modified EC Broth (mEC + n)Composition (g/L)
Tryptone 20.0
Lactose 5.0
No. 3 bile salt 1.12
Dipotassium hydrogen phosphate 4.0
Potassium dihydrogen phosphate 1.5
Sodium chloride 5.0
pH value 6.9 ± 0.1 25 ℃
usage
Weigh 36.62g of this product, heat it and dissolve it in 1000ml of distilled water, divide it into triangular bottles, each bottle is 225ml, autoclaved at 121 ° C for 15 minutes, and after cooling to 50 ° C, add a modified EC broth bacteriostatic agent (containing 4.5mg of neomycin) to every 225ml of medium, mix well and set aside.
Modified EC broth (mEC + n) microbial sensitivity test:
Prepare the medium according to label usage, inoculate the followingquality control strainPlace at 36 ± 1 ° C for aerobic incubation for 18-24 hours.
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
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