Enterococcus Color Developing Medium
[Number]: VIP (HB) 7014-1
[Product name]: Enterococcus color developing medium
[Specifications]: 1000ml
[Purpose]:
[Product name]: Enterococcus color developing medium
[Specifications]: 1000ml
[Purpose]:
Enterococcus Color Developing Medium
Part Number: VIP (HB) 7014-1
Specifications: 1000ml
Product use: for rapid testing of food, water quality, and environmental protectionEnterococcusColony appears red to purple, other bacteria appear colorless, yellow, or suppressed
Enterococcus coloring medium composition (g/L)
Enterococcus coloring medium usage
Weigh 56.4g of this product, heat it in a water bath and dissolve it in 1000ml of distilled water, cool it to 45-50 ° C, pour it into a sterile flat dish, and set aside. No autoclaving required;
Operation steps
Before use, place the prepared medium at room temperature for 10 to 20 minutes.
1. Prepare the sample solution according to national standards, SN standards, FDA standards or other methods.
2. Take 1ml of the sample solution and add it to the enterococcus color-developing medium cooled to 45-50 ° C, mix well or apply it on a plate; or take the sample solution and inoculate it on the enterococcus color-developing medium by scribing;
3. Cultivate at 36 ° C for 24-48 hours, select a plate with 30-200 colonies, and count the red to purple colonies that appear on the plate.
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is light yellow to milky white. The prepared plate was transparent and light yellow medium.
2. Microbial testing
Incubate at 36 ° C for 24-48h:
typical features
Typical colonies range from red to purple.
storage
The prepared plate should be used immediately, and direct light exposure should be avoided. The drying medium should be placed in a dark and dry place, and the storage temperature should be 2-8 ° C, and it should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Microbial sensitivity test of Enterococcus coloring medium:
Prepare the medium according to label usage, inoculate the followingquality control strainPlace at 36 ± 1 ° C for aerobic incubation for 24-48 hours.
Note: When calculating the collection rate, TSA agar was used as the control medium.
Statement: This reference substance and standard is measured by the central inspection: provide website inquiry and purchase services
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