Enterobacter sakazaki coloring medium (
[Number]: VIP (HB) 7012-1
[Product name]: Enterobacter sakazaki color medium (DFI agar)
[Specifications]: 200g
[Purpose]:
[Product name]: Enterobacter sakazaki color medium (DFI agar)
[Specifications]: 200g
[Purpose]:
Enterobacter sakazaki coloring medium (DFI agar)
Part Number: VIP (HB) 7012-1
Specifications: 200g
Purpose: Medium produced according to GB/T 4789.40-2008, used in infant formula and other foodsEnterobacter sakazakiiRapid detection. Enterobacter sakazakii shows blue-green colony, others are colorless or yellow.
Enterobacter sakazakii color developing mediumComposition (g/L)
usage
Weigh 8.62g of this product and add 200ml of distilled water, heat and boil to completely dissolve, autoclave at 121 ° C for 15min, and pour it into a sterile plate when it is cooled to about 50 ° C.
Operation steps
1. Inoculate mLST-Vm broth or EE broth bacterial solution with inoculation ring 10 μL scribing or coating on Enterobacter sakazaki color plate;
2. Put it inverted into an incubator at 36 ° C for 22-26h, and the typical Enterobacter sakazakii is a blue-green colony;
3. Select typical colonies for biochemical testing.
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is light yellow or milky white. The prepared plate was light yellow solid medium.
Microbiological test, incubated at 36 ° C for 22-26 hours:
typical features
The typical Enterobacter sakazakii is a blue-green colony, while the others are colorless, milky white, or yellow.
storage
The prepared plate can be stored for 2-5 days, and direct light exposure should be avoided. The drying medium should be placed in a dark and dry place, the storage temperature should be 2-8 ° C, and it should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Microbial sensitivity test of sakazakii coloring medium 200g:
Prepare the media according to the label usage, inoculate the following quality control strains, and place them at 36 ± 1 ° C for aerobic culture for 22-26 hours.
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