Fraser medium granules
[Number]: VIP (HB) KP4193
[Product name]: Fraser medium granules
[Specifications]: 250g
[Purpose]:
[Product name]: Fraser medium granules
[Specifications]: 250g
[Purpose]:
Fraser medium granules
Name: Fraser Media
Part Number: VIP (HB) KP4193
Specifications: 250g
Purpose: used forListeriaselective growth culture
Remarks: Each bottle needs to be matched with 1 box of VIP (HB) 4190a-1 and 5 boxes of VIP (HB) 4194-2b.
Fraser mediumComposition (g/L)
Tryptone 5.0
peptone 5.0
Beef noodles 5.0
Yeast Powder 5.0
Sodium chloride 20.0
Disodium hydrogen phosphate 12.0
Potassium dihydrogen phosphate 1.35
Aesculin 1.0
Lithium chloride 3.0
pH 7.2 ± 0.2 25 ℃
usage
Weigh 55.0g of this product, heat it and dissolve it in 1000ml of distilled water, autoclave it at 121 ° C for 15 minutes, and cool it to 50 ° C, divide it into 225ml, add 1 FB1 additive A and 1 FB1 additive B to make FB1 (Half-Fraser) medium; divide it into 100ml, add 1 FB2 additive A and 1 Fb2 additive B to make FB2 (Fraser) medium, mix well and set aside.
Bacterial growth in Fraser medium:
In the Fraser medium tube, Listeria monocytogenes and Listeria intoxicum blackened and fluorescence disappeared.
Escherichia coli, Enterococcus faecalis is inhibited and still has fluorescence.
Fraser medium (granules) microbial sensitivity test:
The media was prepared according to the label usage, inoculated with the following quality control strains, and placed at 30 ° C for aerobic culture for 25 ± 1 hour.
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
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