Crystal violet neutral red bile salt agar (
[Number]: VIP (HB) KP0114
[Product name]: Crystal violet neutral red bile salt agar (VRBA) granules
[Specifications]: 250g
[Purpose]:
[Product name]: Crystal violet neutral red bile salt agar (VRBA) granules
[Specifications]: 250g
[Purpose]:
Crystal Violet Neutral Red Bile Salt Agar (VRBA) Granules
Violet Red Bile Agar
Part Number: VIP (HB) KP0114
Specifications: 250g
Purpose: Used for solid plate detection of coliform bacteria
Crystal violet neutral red bile salt agar composition (g/L)
peptone 7.0
Yeast Powder 3.0
Sodium chloride 5.0
Lactose 10.0
Bile salt 1.5
Crystal Violet 0.002
Neutral Red 0.03
Agar 15.0
pH value 7.4 ± 0.1 25 ℃
usage
Weigh 41.5g of this product, heat it and dissolve it in 1000ml distilled water, boil it for no more than 2 minutes. Take 1ml of sample solution with appropriate dilution, add it to the center of a sterile plate, and pour 10-15ml of VRBA cooled to 45 ± 0.5 ° C into the plate. Carefully rotate the plate to fully mix the medium with the sample solution. After solidification, add 3-4ml of VRBA to cover the surface of the plate, turn the plate over, and incubate it at 36 ± 1 ° C for 18-24 hours. No autoclaving is required. Preparation should not exceed 3 hours when used temporarily.
Characteristics of bacterial colonies on crystal violet neutral red bile salt agar (VRBA):
Microbial sensitivity test of crystal violet neutral red bile salt agar (VRBA) granules:
Prepare the medium according to label usage, inoculate the followingquality control strainPlace at 36 ± 1 ° C for aerobic incubation for 18-24 hours.
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
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