UltraBio™ RNA Virus Direct qRT-PCR Preservation Solution

阿拉丁生产的UltraBio™ RNA病毒直接qRT-PCR保存液(UltraBio™ RNA Virus Direct qRT-PCR Preservation Solution)是一种中性、非灭活的病毒保存液，适用于活病毒保存。可用于血浆、血清、无细胞体液、拭子及细胞培养上清等样品采集后的保存和运输，并且无须抽提RNA，可直接用于qRT-PCR，大大简化RNA病毒的核酸检测步骤。本保存液也称为病毒转运液(Viral Transport Medium, VTM)，含有抗生素和FBS。其中抗生素具有广谱抗菌作用，防止加入样品后其它微生物的生长，FBS做为蛋白质稳定剂，对病毒有一定的保护作用，可维持病毒的活性。 使用本保存液保存后的病毒未被灭活，可用于后续病毒的培养和分离。除了常规的非灭活病毒保存液或病毒转运液功能外，在使用合适的一步法qRT-PCR试剂盒情况下，本产品保存的病毒样品也可以直接用做qRT-PCR的模板，大大简化RNA病毒的核酸检测步骤。本产品用于新型冠状病毒(2019-nCoV) N基因慢病毒(新冠假病毒)样品的保存效果如下表所示。一定量新冠假病毒加入咽拭子中并保存在本保存液中，4℃保存72小时，然后直接进行qRT-PCR，与病毒样品置于 使用说明 ： 病毒样本采集后，将病毒样品直接加入含有0.5-1ml RNA病毒直接qRT-PCR保存液的采样管中进行保存，然后置于相应温度条件下保存备用。 Aladdin's UltraBio™ RNA Virus Direct qRT-PCR Preservation Solution is a neutral, non-inactivating solution that can be used for short-term storage and transportation of samples containing live virus, such as plasma, serum, cell-free body fluid, swab and cell culture supernatant after collection. Samples preserved in this product can be directly used for qRT-PCR without RNA isolation.UltraBio™ RNA Virus Direct qRT-PCR Preservation Solution, also called Viral Transport Medium (VTM), contains broad-spectrum antibiotics that can inhibit the growth of other microorganisms, and FBS, a protein stabilizer that can maintain the activity of virus. Viruses preserved in this product are not inactivated and can be used for culture and isolation.Virus sample stored in this product can also be used directly as templates for qRT-PCR, which greatly simplifies the nucleic acid detection of RNA viruses. The following table shows the effect of this product on the preservation of lentivirus of N-gene of novel coronavirus (2019-nCoV). Novel Coronavirus (2019-nCoV) Dual Probes qRT-PCR Kit was used for qRT-PCR analysis. The number of viruses in each PCR reaction was indicated by the Infection Unit (IU). This data is for reference only, which may vary due to different experimental conditions.R0145 (4℃/72h)Positive Control (-80℃)Negative ControlIU/PCR400404400404Sterile waterCt Value124.9529.2632.3124.7128.5531.88Undetermined224.8829.5032.1624.9429.0231.89Undetermined324.8929.3632.2524.8928.8931.89UndeterminedAverage24.9129.3732.2424.8428.8231.89-RNA in samples preserved with this product can be extracted directly with Trizol or spin columns such as Aladdin's RNAeasy™ Viral RNA Isolation Kit with Spin Column .Samples preserved in this product should be tested within 72h and transported at 2-8℃. For long-term storage, samples can be stored at -80℃ in this product or preserved in UltraBio™ Viral RNA Stable Preservation Solution .R0145-100ml is sufficient for preserving 100-200 samples and R0145-500ml is sufficient for preserving 500-1000 samples. Precautions ： Specimen collectors need to be properly protected according to the relevant requirements, and subsequent operations should be performed in a Class II biosafety cabinet, following the Class II biosafety or higher standard. Fast™ SYBR Green One-Step qRT-PCR Kit , Fast ™ Probe One-Step qRT-PCR Kit or other validated kits are recommended for direct qRT-PCR analysis of preserved samples without RNA extraction. However, due to the diversity and uncertainty of the viral samples, we recommend verifying the analysis result with isolated RNA.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use ： After sample collection, put the sample directly into a sampling tube containing 0.5-1ml of this product, and then store at an appropriate temperature for future use.

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