网织红细胞是未完全成熟的红细胞，由于其胞浆中尚残有核糖体等嗜碱性物质，经煌焦油蓝染液染色后，可见有蓝绿色的点状、线状或网状结构，网织红细胞点状结构越多，表示细胞越年轻，反之则越成熟。 操作流程： 1 样品处理 1.1 对于石蜡切片： 1.1.1 用二甲苯脱蜡，2×5~10min； 1.1.2 100%乙醇水化 5min，95%乙醇 2min，70%乙醇 2min；1.1.3 PBS 漂洗 2min。 1.2 对于冰冻切片： 1.2.1 PBS 漂洗 2min。 1.3 对于培养细胞： 1.3.1 用 4%多聚甲醛固定 10 min 以上； 1.3.2 PBS 漂洗 2×2 min； 2 煌焦油蓝染液染色 对于上述处理好的样品，煌焦油蓝染液染 5-30min。 3 脱水、透明并封片。 无水乙醇直接分化与脱水，3~5min；二甲苯透明 3~5min，中性树胶封固。 注意事项： 1 需自备 4%多聚甲醛、梯度乙醇、二甲苯，中性树胶或其它封片剂。 2 第一次使用本试剂盒时建议先取 1~2 个样品做预实验。 温馨提示： 为了您的自身安全，使用试剂前，请做好防护，如穿实验服，带手套等。 Reticulocytes are immature red blood cells. Due to the residual basophilic substances such as ribosomes in the cytoplasm, blue-green dot, linear or reticular structures can be seen after staining with brilliant tar blue solution. The more dot structures of reticulocytes, the younger the cell is, and the more mature the cell is. Operation process: 1 Sample handling 1.1 For paraffin sections: 1.1.1 Dewaxing with xylene, 2×5~10min; 1.1.2 100% ethanol hydration for 5min, 95% ethanol for 2min, 70% ethanol for 2min; 1.1.3 Rinse with PBS for 2min. 1.2 For frozen sections: 1.2.1 Rinse with PBS for 2min. 1.3 For cultured cells: 1.3.1 Fixed with 4% paraformaldehyde for more than 10 minutes; 1.3.2 Rinse with PBS for 2×2 min; 2 Brilliant tar Blue dyeing solution For the above treated samples, brilliant tar blue dyeing solution for 5-30min. 3 Dehydrate, transparent and seal. Direct differentiation and dehydration of anhydrous ethanol, 3~5min; Xylene transparent 3~5min, neutral gum seal. Note: 1 Need to bring 4% paraformaldehyde, gradient ethanol, xylene, neutral gum or other sealing tablets. 2 When using this kit for the first time, it is recommended to take 1 to 2 samples for pre-test. Tips: For your own safety, please take good protection before using the reagent, such as wearing a lab coat, wearing gloves, etc.
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Brilliant Cresyl Blue solution (1%)
CAS number：unknown molecular formula：
overview
compound introduction
Specs
| Chinese alias | 煌焦油蓝染液 | ||
| English alias | Brilliant Cresyl Blue solution | Brilliant Tar Blue Staining Solution | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | BioReagent |
| PSA | - | logp | - |
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