Ham's F-12 培养基(Ham's F-12 Nutrient Mixture)是由 Ham 在 1969 年以 Ham's F-10 复合营养培养基为基础设计而成的,最初用于 CHO 细胞的无血清培养。Ham's F-12 含有一些传统基础培养基没有的成分,如腐胺、次黄嘌呤、胸苷、锌等,常作为无血清培养的基础培养液,在低血清含量下时,特别适合单细胞培养和克隆化培养,添加血清后也广泛应用于癌细胞和原代细胞的培养,如大鼠肝细胞、大鼠前列腺上皮细胞、软骨细胞、大鼠成肌细胞、鸡胚胎细胞等。此外,Ham's F-12 与 DMEM 等体积混合后,得到的 DMEM/F12 培养基营养更为丰富,使用更为广泛。 本产品含有:L-谷氨酰胺、酚红、丙酮酸钠(110mg/L)。 不含有:HEPES、双抗。
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Ham's F-12 Nutrient Mixture
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| Chinese alias | Ham's F-12 营养培养基 | F-12培养基 (with L-glutamine) | 营养混合物 F-12 Ham | ||
| English alias | Ham's F-12 Nutrient Mix | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无菌过滤 |
| PSA | - | logp | - |
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