DyLight™ 405 Maleimide

Product introduction： DyLight 405 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 405 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes. has high fluorescence intensity over a broad pH range (pH 4 - 9) and is more photostable than Alexa Fluor™ 405, Cascade™ Blue and AMCA dyes in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. Features of DyLight 405 Maleimide: • High performance— DyLight 405 shows brighter fluorescence than Alexa Fluor 405, Cascade Blue and AMCA • Specific— maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH) • Efficient labeling methods— well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling • Optimized antibody labeling procedure— complete protocol for IgG reduction and labeling and calculating the labeling efficiency Applications: • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies DyLight 405 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5 - 7.5. Learn more about maleimide chemistry.

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