阿拉丁生产的TBSTw (TBS with Tween-20),可以用于免疫印迹(Immunoblot, 即Western blot或WB)、免疫荧光(Immunofluorescence, IF)、免疫组化(Immunohistochemistry, IHC)和免疫细胞化学(Immunocytochemistry, IC)等实验中封闭液的配制、一抗或二抗的配制、一抗或二抗孵育后的洗涤等。封闭和洗涤可以降低背景,增强信噪比。本产品TBSTw为1X即用型溶液,含有20 mM Tris, 137 mM NaCl, 0.1% Tween-20,pH7.6±0.1 (25℃)。对于Western blot实验中膜的洗涤,按照每张膜洗涤需要5-10毫升TBSTw计算,一个包装的本产品可以洗涤50-100次;对于免疫染色等实验,按照每个样品洗涤需要0.2-1毫升TBSTw (1X)计算,一个包装的本产品可以洗涤500-2500个样品。4℃保存,一年有效。-20℃可以长期保存,室温保存两周内有效。本产品经过滤除菌处理,使用过程中应尽量避免细菌污染。一旦启用后建议在2周内用完或者把剩余溶液冷冻保存。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。为了您的安全和健康,请穿实验服并戴一次性手套操作。
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TBSTw (TBS with Tween-20)
CAS number:unknown molecular formula:
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| Chinese alias | - | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 1X |
| PSA | - | logp | - |
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