应用 PC 生物素-NHS 酯是一种独特的胺反应性、可光裂解的生物素试剂，可从链亲和素中无试剂释放捕获的生物分子。该试剂包含一个生物素部分，该生物素部分通过含有光可裂解部分的间隔臂与胺反应性N-羟基琥珀酰亚胺基（NHS）混合碳酸盐连接。该化合物的 NHS 部分与目标分子上的伯胺基团特异性反应，形成氨基甲酸酯键。使用便宜的近紫外低强度灯（例如 1-5 mW/cm2的 365 nm 灯），可以在 5-25 分钟内有效地光致释放捕获的生物分子，通常 >90%。 Description PC biotin-NHS ester is a unique amine-reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to the amine reactiveN-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).
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PC biotin-PEG3-NHS ester
CAS number：2353409-93-3 molecular formula：C36H52N6O15S
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compound introduction
Specs
| Chinese alias | - | ||
| English alias | PC biotin-NHS ester | ||
| CAS number | 2353409-93-3 | molecular formula | C36H52N6O15S |
| molecular weight | 840.89 g/mol | Exact mass | ≥96% |
| PSA | - | logp | - |
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2353409-93-3 | PC Biotin-PEG3-NHS Ester.pdf
