aladdin® SE (or NHS ester)是我司生产的具有氨基反应活性的一类荧光染料。该类染料的SE基团可以与氨基基团反应生产稳定的酰胺键。相比于市面上其他同类染料,aladdin®是稳定性更强、水溶性更好、荧光强度更优的新一代荧光染料。 我司还提供小批量抗体标记试剂盒aladdin® Antibody Labeling Kits(S6011),可在30 min内标记5-100 μg抗体,而无需纯化步骤,简单方便。 注意事项: 1. 标记的蛋白如需长期储存,推荐加入5-10 mg/mL BSA和0.01-0.03% NaN3,以防止蛋白变性和微生物滋生。放置于4℃避光保存。若加入了终浓度为50%的甘油,可放-20℃保存。可稳定保存一年以上。 2. 操作过程注意避光,搅拌速度应适当以避免产生气泡。 3. 层析柱装柱时,尽量使柱体均匀,柱面平整,无气泡、裂隙。 4. 上样时注意,当柱顶缓冲液与凝胶平面相切时再加样品,洗脱时,当样品走至与凝胶平面相切时再加洗脱液。 5. 影响标记效率的其他因素还包括:温度、反应时间、pH、荧光染料与蛋白的量等,需注意控制。 6. 为了您的安全和健康,请穿实验服并戴一次性手套。 应用范围: 蛋白核酸标记染料 Product introduction: Aladdin ® SE (or NHS ester) is a kind of fluorescent dye with amino reaction activity produced by our company.SE groups of these dyes can react with amino groups to produce stable amide bonds. Compared with other similar dyes on the market, Aladdin ® It is a new generation of fluorescent dyes with stronger stability, better water solubility and better fluorescence intensity. Our company also provides a small batch of antibody labeling kit Aladdin ® Antibody labeling kits (s6011), which can label 5-100 in 30 min μ G antibody without purification steps, which is simple and convenient. Matters needing attention: 1. if the labeled protein needs long-term storage, it is recommended to add 5-10 mg/ml BSA and 0.01-0.03% NaN3 to prevent protein denaturation and microbial breeding. Store at 4 ℃ away from light. If glycerol with a final concentration of 50% is added, it can be stored at -20 ℃. It can be stably stored for more than one year. 2. keep away from light during operation. The mixing speed should be appropriate to avoid bubbles. 3. when installing the chromatographic column, try to make the column body uniform, the column surface flat, and free of bubbles and cracks. 4. pay attention to adding the sample when the column top buffer is tangent to the gel plane. When eluting, add the eluent when the sample is tangent to the gel plane. 5. other factors affecting the labeling efficiency also include temperature, reaction time, pH, the amount of fluorescent dye and protein, etc., which should be controlled. 6. for your safety and health, please wear laboratory clothes and disposable gloves. Scope of application: Protein nucleic acid labeling dye
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Aladdin ® 640-5SE (Aladdin ® 640-5 succinimidyl ester)
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| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | - |
| PSA | - | logp | - |
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