来源于大肠杆菌重组表达的热稳定无机焦磷酸水解酶(Inorganic Pyrophosphatase),可以催化一分子焦磷酸盐转化为两分子磷酸盐离子。这种驱动生成焦磷酸盐的代谢反应是一个高放能的反应,因此该反应可偶联到一些热力学上不利的转化,以便驱动这些转化进行到完全。热稳定无机焦磷酸酶(PPase)催化无机焦磷酸盐水解生成正磷酸盐。在分子生物学中可用于反转录反应中提高RNA产量、DNA扩增中提高DNA合成效率。 用 途 催化PPi + H2O→2Pi 作为PCR、RPA混合物的组分。 推荐使用条件 储存缓冲液:20 mmol/L Tris-HCl (pH 8.0), 100 mmol/L NaCl, 0.1 mmol/L EDTA, 1mmol/L DTT, 50%(v/v)甘油。
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热稳定无机焦磷酸酶
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | Pyrophosphatase Inorganic | inorganic diphosphatase | diphosphate phosphohydrolase | inorganic pyrophosphatase | pyrophosphate phosphohydrolase | ppa | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 生物活性 |
| PSA | - | logp | - |
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