D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent. In Vitro D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay. D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM. D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment. D-3 has little influence on various non-iPSCs, including hepatocytes and neurons. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Six human iPSCs lines and one human ESC line (khES1). Concentration: 400 μM. Incubation Time: 1-2 h. Result: Sufficient to induce a viability loss (>99% in all iPSC lines and >95% in khES1). Form:Solid
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D-3
CAS number：1967815-98-0 molecular formula：C48H47N4O10P
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| Chinese alias | - | ||
| English alias | - | ||
| CAS number | 1967815-98-0 | molecular formula | C48H47N4O10P |
| molecular weight | 870.88 g/mol | Exact mass | ≥99% |
| PSA | - | logp | - |
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