Glycan Sequencing Kit

Product Description Our Glycan Sequencing Kit includes the enzymes and buffer required to sequence ten N-linked oligosaccharides. Contents Neuraminidase from Arthrobacter ureafaciens – 80 µl Beta-Galactosidase from Streptococcus pneumoniae – 60 µl N-Acetylglucosaminidase from Streptococcus pneumoniae) – 40 µl Alpha-Mannosidase from Jack Bean – 20 µl Core Alpha-Mannosidase from X. manihotis) – 10 µl 5X Reaction buffer – 400 µl Analysis Many methods of analysis are available, including HPLC, gel electrophoresis, HPAEC, capillary electrophoresis, and mass spectrometry. For more information on these methods, please contact us. Stability The Glycan Sequencing Kit is stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. Purity All Enzymes are tested for contaminating protease by incubating 10 μg of denatured BSA with 2 μl of enzyme at 37°C for 24 hours. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strains for our recombinant enzymes have been extensively tested and do not produce any detectable glycosidases. Enzymes purified from native sources are tested for contaminating exoglycosidases The absence of exoglycosidase contaminants is confirmed by extended incubations with the corresponding pNP-glycosides.

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