PNGase F全称肽 N-糖苷酶F,基因来源于Elizabethkingia miricola。PNGase F 是一种胺酶,切割糖蛋白上的 N-连接糖链。切割的糖型有:高甘露糖、杂合和复杂寡糖;切割位点为:最内侧的 N-乙酰葡糖胺和天冬酰胺之间的糖苷键,能够将绝大多数 N-糖链(除含 α-1,3 连接的核心岩藻糖)。适用于蛋白质组学及糖生物学研究中糖蛋白上 N-连寡糖链的有效释放。 反应式 最佳反应条件: 最适反应 pH:7.5;热失活条件:75℃处理10 min。 组分和说明 P755115
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重组糖基肽酶F
CAS number:unknown molecular formula:
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| Chinese alias | N-糖苷酶F | 糖苷酶F | 肽N-糖苷酶 | ||
| English alias | N-Glycosidase F | Peptide N-glycosidase | peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase | glycopeptidase | glycopeptide N-glycosidase | N-glycanase | N-oligosaccharide glycopeptidase | ngl | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 生物活性 |
| PSA | - | logp | - |
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