重组PNGase F经亲和色谱纯化后，透析到pH 7.5、含10 mM磷酸钾的50%甘油溶液中，得到稳定的产物。该产品含有少量缓冲盐。这种高度纯化的材料可用于制备性去糖基化或用于分析凝胶、溶液或印迹膜。可利用该酶C端的6x组氨酸融合标签而将其从制备性操作中去除。来源于Elizabethkingia meningoseptica的PNGase F可用于人血浆样品的去糖基化分析和硫酸软骨素蛋白聚糖的去糖基化。用于使蛋白质去糖基化。 Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan. Used to deglycosylate protein.
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PNGase F脑膜炎沙门氏菌
CAS number：unknown molecular formula：
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| Chinese alias | - | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 重组 |
| PSA | - | logp | - |
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