硫酸软骨素酶AC (Chondroitinase AC) 来源于肝素黄杆菌(Flavobacterium heparinum),可降解硫酸软骨素A、硫酸软骨素C。此酶通过β消除机理作用于二糖重复单元之间的β1-4糖苷上并将其切断。降解反应的产物为寡糖,主要是不饱和二糖。 用途: 酶解 HPLC法测定硫酸软骨素含量和二糖组成;低分子硫酸软骨素酶法制备;肝素、硫酸乙酰肝素的精制。 推荐使用条件: 最适pH:8.0 pH适用范围:5.5-9.5 最适温度:37 ℃ 温度适用范围:10~70 ℃ 使用缓冲液:20mmol/L Tris-HCl (pH:8.0),150 mmol/L NaCl, 5mmol/L MgCl2
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软骨素酶AC
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 软骨素酶AC | 硫酸软骨素AC酶 | 硫酸软骨素酶AC | ||
| English alias | chondroitin AC lyase | chondroitin AC eliminase | chondroitinase | chondroitin sulfate lyase | cslA | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 生物活性 |
| PSA | - | logp | - |
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