产品介绍 SDS-PAGE蛋白上样缓冲液（还原性，2X）是以溴酚蓝为染料，2倍浓缩的SDS-PAGE 凝胶电泳上样缓冲液，具有沉淀蛋白样品进入凝胶孔和指示蛋白样品在凝胶中运动位置的作用，同时缓冲液中含有 DTT/B-巯基乙醇和 SDS，可使蛋白质分子的链内二硫键和链间二硫键断裂,各蛋白亚单位彼此分离以及蛋白变性的作用，适用于还原型 SDS-PAGE 的蛋白样品制备和上样。 注意事项： 1. 加样前在室温或 37-40℃ 数分钟解冻后轻轻摇匀，以确保溶液混合均匀; 2.本试剂含有β-巯基乙醇，操作时请穿着实验服并佩戴一次性手套; 3. 本产品仅用于科研，不能用于人体实验或人体治疗; 4. 为了您的自身安全，使用试剂前，请做好防护，如穿实验服，带手套等。 使用说明： 1、试剂准备 1.1将蛋白示踪上样缓冲液（2×还原）与蛋白样品按照1:1的比例混匀； 2、蛋白样品处理 2.1将蛋白样品置于沸水浴中加热3-5分钟； 2.2待蛋白样品充分变性后冷却至室温，以小于3000rpm的条件离心30秒； 3、蛋白上样及电泳 3.1离心后，以微量进样器取适量上清，直接加入SDS-PAGE凝胶加样孔内； 3.2进行常规电泳，通常染料到达距离凝胶底端0.5cm-1cm处即可停止电泳。 Product Introduction: The protein tracer sample loading buffer (2x reduction) is a double concentrated SDS-PAGE gel electrophoresis sample loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into the gel pores and indicating the movement position of protein samples in the gel. At the same time, the buffer contains DTT/B-mercaptoethanol and SDS, which can break the disulfide bond within and between the chains of protein molecules, separate each protein subunit from each other, and denature the protein. It is suitable for the preparation and sample loading of reduced SDS-PAGE protein samples. Precautions： 1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution; 2. This reagent contains β - mercaptoethanol. Please wear experimental clothing and disposable gloves when operating; 3. This product is only used for scientific research and cannot be used for human experiments or treatments; 4. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents. Instructions for Use： 1. Reagent preparation 1.1 Mix the protein tracer loading buffer (2 x reduction) with the protein sample in a 1:1 ratio; 2. Protein sample processing 2.1 Heat the protein sample in a boiling water bath for 3-5 minutes; 2.2 After sufficient denaturation of the protein sample, cool it to room temperature and centrifuge at less than 3000 rpm for 30 seconds; 3. Protein loading and electrophoresis 3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole; 3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.
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SDS-PAGE Sample Loading Buffer (Reduced, 2X)
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| Chinese alias | SDS-PAGE蛋白上样缓冲液 | SDS-PAGE蛋白上样缓冲液(还原性, 2X) | 变性还原PAGE蛋白上样缓冲液 | 2×SDS-PAGE蛋白上样缓冲液 | ||
| English alias | SDS-PAGE Sample Loading Buffer, 2X | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | BioReagent |
| PSA | - | logp | - |
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