过氧化物酶（POD），已激活

激活的过氧化物酶（POD）已被用于末端脱氧核糖核苷酸转移酶（TDT）介导的dUTP-地高辛缺口末端标记（TUNEL）染色 和免疫组化。该酶无需预先活化即可用于标记具有反应性和可反应伯氨基的水溶性物质（例如，肽或蛋白质），其可与过氧化物酶一起用于不同分析方法中。它特别适用于将抗体（来自兔、小鼠、绵羊和山羊的Ig、Ig Fab和Ig F（ab′）2片段）与过氧化氢酶耦合。所得的缀合物最适合用于免疫化学检测系统，例如ELISA、免疫组织化学和免疫印迹过程。应用： 供体：过氧化氢氧化还原酶作为试剂用于与辣根过氧化物酶（HRP）一起标记带有伯氨基的水溶性物质。容量：足够用于约6 mg IgG。过氧化物酶（POD）包括一组特定的酶，例如NAD过氧化物酶、NADP过氧化物酶、脂肪酸过氧化物酶和一组来自多种来源的非特定酶。它存在于动物、高等植物和其他生物中。 Donor: hydrogen-peroxide oxidoreductase Reagent for the labeling of water-soluble substances carrying primary amino groups with peroxidase from horseradish (HRP). Capacity : sufficient for approximately 6 mg IgG. Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as NAD peroxidase, NADP peroxidase, fattyacid peroxidase and a group of non specific enzymes from various sources. It is present in animals, higher plants and other organisms. Application: Peroxidase (POD), activated has been used in terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) staining and immunohistochemistry. The enzyme can be used without pre-activation for labeling water-soluble substances with reactive and accessible primary amino groups ( e.g. , peptides or proteins) with peroxidase for use in analytical methods. It is particularly suitable for the coupling of antibodies (Ig, Ig Fab, and Ig F(ab′) 2 fragments from rabbit, mouse, sheep, and goat) with peroxidase. The resulting conjugate is optimal for use in immunochemical detection systems, for example, ELISA, immunohistochemistry, and immunoblotting procedures.

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