TMB是辣根过氧化物酶的底物。其可形成可溶性蓝色终产物，并可在370nm或655nm下读数。可以用0.5M H2SO4停止TMB反应（产生黄色溶液），并在450nm处读取数值。推荐用于ELISA（微孔）分析，不建议用于膜应用。通过将测量的底物吸收值超过吸收最大值，3,3′,5,5′-四甲基联苯胺已在研究中用于证明，任何免疫酶测定的测量范围都可以被显著延长，而又不损害测定特征。3,3′,5,5′-四甲基联苯胺也已用于研究中，以了解具有过氧化物酶样活性的CuS凹面上层结构的形成。 TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H 2 SO 4 , resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
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3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物体系
CAS number：unknown molecular formula：
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | peroxidase substrate |
| PSA | - | logp | - |
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