NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed. In Vitro NSP-SA-NHS marking? (1) 1mg BSA was dissolved in 0.3 mL PBS (solution A), and 0.2 mg NSP-SA-NHS was dissolved in 40 μL DMF (solution B). ? (2) Add solution B to solution A, mix at 18°C, 130 rpm, and dark for 12 h. ? (3) The mixture was dialyzed with 500 mL of distilled water at 4°C for 2 d, during which distilled water was changed twice (solution C). ? (4) Slowly add solution B to solution C, mix at 18°C, 130 rpm, and dark for 24 h, and then dialysis with distilled water for 48 h. During this period, change distilled water four times. ? (5) Dilute the coupling compound with distilled water to a suitable concentration, and analyze it with an ultraviolet spectrophotometer. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid
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NSP-SA-NHS
CAS number：199293-83-9 molecular formula：C32H31N3O10S2
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| Chinese alias | 3-(9-((4-((2,5-二氧代吡咯烷-1-基)氧基)-4-氧代丁基)(对甲苯磺酰基)吖啶-10-鎓-10-基)丙烷-1-磺酸内盐 | 吖啶磺酰胺 | ||
| English alias | 3-(9-((4-((2,5-Dioxopyrrolidin-1-yl)oxy)-4-oxobutyl)(tosyl)carbamoyl)acridin-10-ium-10-yl)propane-1-sulfonate | ||
| CAS number | 199293-83-9 | molecular formula | C32H31N3O10S2 |
| molecular weight | 681.73 g/mol | Exact mass | ≥98% |
| PSA | 196.000 Ų | logp | 3.000 |
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