储存缓冲液: 50mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, 0.1% Triton X-100(v/v), 50% Glycerol(v/v), pH 7.5 产品介绍: RNase R(Ribonuclease R)是一种来源于大肠杆菌 RNR 超家族且依赖 Mg 2+的核糖核酸外切酶,从3'→5'方向将线性RNA完全水解成二核苷酸和三核苷酸。对环状 RNA 及双链 RNA 不敏感,可用于特殊结构 RNA 的生产,如环状RNA(circRNA)、套索结构 RNA(lariat RNA)、3'突出末端少于 7 个核苷酸的双链 RNA 以及具有复杂结构的 tRNA 等。RNase R常用于基因表达和可变剪切研究,可消化线性 RNA 使 circ RNA 或 lariat RNA 得到富集。 来源 重组 E.coli 活性单位定义 在 37℃标准反应条件下, 10min 将 1μg poly(A)转化为酸溶性核苷酸所需的酶量定义为1U 推荐 RNase R 消化体系: Component
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RNase R
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 核糖核酸酶 | 高效 RNase R核酸外切酶 | ||
| English alias | RNase R | Ribonuclease R | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | BioReagent |
| PSA | - | logp | - |
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