Gram′s crystal violet solution

结晶 紫常用于革兰氏染色及其变体、 染色淀粉样物质、细菌成分和维管植物组织。它用于环氧树脂切片的多色染色、 培养神经元的活性染色以及分析减数分裂结构的 共聚焦光学切片。它还被用于细胞内细菌、微孢子虫孢子和细胞学涂片的 吖啶橙-结晶紫染色。应用： 革兰氏结晶紫溶液，也称为龙胆紫或六甲基副玫瑰苯胺，是一种基本、阳离子、嗜酸性氨基芳甲烷染料。它是六-N-甲基化的三氨基三苯甲烷，具有中等大小的亲脂性阳离子和轻微非平面的共轭体系。在细菌学中，它用作革兰氏染色的主要染料，以区分革兰氏阳性菌和革兰氏阴性菌。众所周知，它具有抗菌和抗真菌的特性。 Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties. Application: Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues. It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures. It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.

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