MEM

低限量 Eagle 培养基已用于小鼠成纤维细胞培养，用于体外细胞相容性测试。应用： 由 Harry Eagle 开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。由 Harry Eagle 开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。正常哺乳动物成纤维细胞和某些亚型的 HeLa 细胞的早期尝试培养表明,Eagle 基础培养基（BME）无法满足的他们的特殊营养需求。随后培养这些和其他细胞的研究表明，在 BME 中补充添加剂有利于更多种类的苛求细胞的生长。MEM 包含了这些改进，包括更高浓度的氨基酸，因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸，扩大了该培养基的用途。 Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Application: Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

No numbering system information yet

No physical and chemical property information yet

No safety information yet

No production method and use information yet

No upstream information yet

No downstream information yet

No related product information yet