类器官传代消化液可应用于肿瘤或正常类器官的常规传代,能将类器官器官从基质胶中分离,并温和地消化为寡细胞团或单细胞,同时保持其传代后的生长活力。 使用说明: 1、用1mL移液枪头刮取含类器官的基质胶团,与液体一起转入离心管中,轻轻吹打几次,300g离心3min,小心吸弃上清。 注:为避免枪头粘附性损耗,可将1mL移液枪头反复吹吸抗黏附液,以润洗枪头。 2、加入5mL类器官传代消化液,37℃恒温消化5-10min,期间颠倒混匀若干次。 注:在消化过程中,可使用移液器吹打混匀帮助消化。也可实时取少量消化悬液于显微镜下观察消化情况,当观察到较多的单细胞或直径在50μm以下的细胞簇后,即可认为消化完成。 3、加入5mL基础培养基(或含10% FBS的基础培养基)重悬,300g离心3min,小心吸弃上清液。 4、加入5mL基础培养基(或含10% FBS的基础培养基)重悬,300g离心3min,小心吸弃上清液。 5、将上述获得的类器官用于后续类器官培养、冻存等试验操作。 是否无菌:是
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Organoid Dissociation Solution
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 类器官传代消化液 | ||
| English alias | Organoid Dissociation Solution | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无菌过滤 |
| PSA | - | logp | - |
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