Product introduction： DyLight 594 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 594 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes. provides vibrant orange-to-red fluorescence with better performance than other rhodamine derivatives including Alexa Fluor™ 594 and Texas Red™ dye for fluorescent applications over a broad pH range (pH 4 - 9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. Features of DyLight 594 Maleimide: • High performance— DyLight 594 shows brighter fluorescence than Alexa Fluor 594 and Texas Red • Specific— maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH) • Efficient labeling methods— well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling • Optimized antibody labeling procedure— complete protocol for IgG reduction and labeling and calculating the labeling efficiency Applications: • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies DyLight 594 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more aboutmaleimide chemistry.
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DyLight™ 594 Maleimide
CAS number：unknown molecular formula：
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| Chinese alias | DyLight™ 594 马来酰亚胺 | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | - |
| PSA | - | logp | - |
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