EMSA突变探针－AP1是用于EMSA(也称gel shift)研究的AP1 consensus oligonucleotide的突变体。可以作为EMSA探针－AP1的阴性对照，用于EMSA结合反应中突变探针的冷竞争反应等。 EMSA突变探针－AP1的序列如下：5’-CGC TTG ATG ACT TGG CCG GAA-3’3’-GCG AAC TAC TGA ACC GGC CTT-5’', EMSA突变探针－AP1中AP1的公认的结合位点发生了突变，从而使AP1无法和该突变探针结合。在探针冷竞争反应中，正常的标记探针和AP1的结合的条带会被抑制；而在突变探针冷竞争反应(cold competition)中，正常的标记探针和AP1的结合的条带不会被抑制。参考下图。1,阴性对照反应(标记探针)；2,常规反应(含激活的目的转录因子的核蛋白＋标记探针)；3,探针冷竞争反应(含激活的目的转录因子的核蛋白＋标记探针＋标记探针100倍量的未标记探针)；4,突变探针的冷竞争反应(含激活的目的转录因子的核蛋白＋标记探针＋标记探针100倍量的未标记突变探针)；5,Super-shift反应(含激活的目的转录因子的核蛋白＋标记探针＋目的转录因子的特异抗体)。 一个包装的突变探针，如果用于同位素标记EMSA探针的突变探针冷竞争反应，可以进行90-180个突变探针的冷竞争反应。如果用于生物素标记探针的冷竞争时，可以进行约30个冷竞争反应。 注意事项 ： 避免加热到40℃以上，温度过高会导致双链DNA探针解聚成单链。而单链无法用于EMSA研究。对于EMSA的详细操作可以参考我们的EMSA试剂盒的使用说明。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 ： 1. 用于突变探针的冷竞争反应，可以参考如下反应体系：突变探针的冷竞争反应： Nuclease-Free Water4μlEMSA/Gel-Shift结合缓冲液(5X)2μl细胞核蛋白或纯化的转录因子2μl未标记的突变探针1μl标记好的探针1μl总体积10μl如果使用更大体积的未标记的突变探针，Nuclease-Free Water的体积就要相应减少，使总体积保持不变。 Aladdin's EMSA Mutant Probe - AP1 is a mutant of the AP1 consensus binding oligonucleotide designed for EMSA . It can be used as a negative control of EMSA Probe - AP1in cold mutant probe competition reactions.The nucleotide sequence of EMSA Mutant Probe - AP1 is as follows Precautions ： Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kits from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use ： 1.Set up the cold mutant probe competition reaction by adding the reagents in sequence as follows:Nuclease-free Water 4µlEMSA/Gel-Shift Binding Buffer (5X) 2µlNuclear proteins or purified transcription factors 2µlUnlabeled mutant probe 1µlLabeled probe 1µlTotal volume10µl Note: If a larger volume of unlabeled mutant probe is used, the volume of Nuclease-free Water should be reduced accordingly to keep the total reaction volume at 10µl.
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Mutant EMSA Probe AP1
CAS number：unknown molecular formula：
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Specs
| Chinese alias | - | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 10μM |
| PSA | - | logp | - |
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