产品介绍： EDTA抗原修复液(50×)采用了广泛使用的EDTA，具有修复多聚甲醛等固定液引起的蛋白交联，无论石蜡切片、冰冻切片或者细胞爬片，只要是用多聚甲醛、甲醛或其它醛类试剂固定的样品，进行抗原修复都会有效去除蛋白之间的交联，充分暴露抗原表位，从而大大改善免疫染色效果。 操作流程： 1、试剂准备使用前须用蒸馏水或超纯水稀释EDTA抗原修复液(50×)，配成抗原修复液(1×)。 2、抗原修复 2.1对于石蜡切片： 2.1.1脱蜡：切片在二甲苯中脱蜡2×5min。无水乙醇2×5min。90%乙醇2×5min，70％乙醇5min。蒸馏水2×5min。 2.1.2抗原修复：将切片浸泡于抗原修复液(1×)中，95-100°C加热约10~30min(最佳的加热时间需根据不同的样品和目的蛋白自行摸索)。 注：抗原修复液(1×)使用前需预热到95-100°C。加热可以使用水浴锅或微波炉。如果使用微波炉加热，需注意避免修复液暴沸。随后大约在20~30min冷却至室温。用免疫染色洗涤液洗涤1~2次，每次3~5min。即可进行封闭等后续的免疫染色步骤。 2.2冰冻切片：用免疫染色洗涤液洗涤切片5min。抗原修复的过程如石蜡切片的抗原修复过程。 2.3其它样品的抗原修复，可以参考石蜡切片的步骤进行。 温馨提示：为了您的自身安全，使用试剂前，请做好防护，如穿实验服，带手套等。 Product Introduction: The EDTA antigen repair solution (50x) uses widely used EDTA, which has the ability to repair protein crosslinks caused by fixatives such as paraformaldehyde. Whether it is paraffin sections, frozen sections, or cell slides, as long as the sample is fixed with paraformaldehyde, formaldehyde, or other aldehyde reagents, antigen repair will effectively remove protein crosslinks, fully expose antigen epitopes, and greatly improve immunostaining efficiency. Operation process: 1. Before preparing the reagent for use, it is necessary to dilute EDTA antigen retrieval solution (50 x) with distilled water or ultrapure water to prepare an antigen retrieval solution (1 x). 2. Antigen repair 2.1 For paraffin sections: 2.1.1 Dewaxing: The slices are dewaxed in xylene for 2 × 5 minutes. Anhydrous ethanol for 2 × 5 minutes. 90% ethanol for 2 × 5 minutes, 70% ethanol for 5 minutes. Distilled water for 2 × 5 minutes. 2.1.2 Antigen retrieval: Soak the slices in antigen retrieval solution (1 ×) and heat them at 95-100 ° C for about 10-30 minutes (the optimal heating time needs to be explored according to different samples and target proteins). Note: The antigen repair solution (1 ×) needs to be preheated to 95-100 ° C before use. Heating can be done using a water bath or microwave. If using a microwave oven for heating, be careful to avoid boiling the repair solution. Then cool to room temperature for approximately 20-30 minutes. Wash 1-2 times with immunostaining detergent, each time for 3-5 minutes. Subsequent immunostaining steps such as sealing can be performed. 2.2 Frozen sections: Wash the sections with immunostaining detergent for 5 minutes. The process of antigen repair is similar to the antigen repair process of paraffin sections. 2.3 For antigen repair of other samples, the steps for paraffin sectioning can be followed. Reminder: For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.
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EDTA Antigen Retrieval Solution (50X)
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| Chinese alias | EDTA抗原修复液 | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无菌过滤 |
| PSA | - | logp | - |
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