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β-1,4-半乳糖基转移酶1

CAS number:unknown    molecular formula:

overview

compound introduction

该重组B4GALT1产物可用于研究该酶的作用方式及其潜在的抑制剂。它还可用作体外修饰糖蛋白的糖工程工具。应用: 重组人β-1,4-半乳糖基转移酶1(B4GALT1)在人HEK 293细胞中表达为糖蛋白,计算分子量为40 kDa。由于糖基化作用,DTT还原蛋白在SDS-PAGE上以~55 kDa多肽形式迁移。这种蛋白质是在人细胞中产生的,不含血清。人细胞表达系统允许类人糖基化和折叠,通常支持比活性更高的蛋白质。 Recombinant human Beta-1,4-galactosyltransferase 1 (B4GALT1) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 40 kDa. The DTT-reduced protein migrates as a ~55 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. Application: This recombinant B4GALT1 product can be used to study the mode of action of the enzyme, as well as its potential inhibitors. It can also be used as a glycoengineering tool to modify glycoproteins in vitro.

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