Lectin from Arachis hypogaea (peanut)

花生凝集素已用于：测定活性精子细胞的顶体状态（存在或不存在顶体基质，对应完整或已经过顶体反应的精子）荧光染料标记PNA和B220抗体探测纵隔淋巴结(mLN)冷冻切片以检测生发中心(GC)可视化预处理视网膜切片的锥体外节应用： 凝集素是碳水化合物结合蛋白，广泛存在于真菌、植物和动物中。植物和动物凝集素的结构经过了各种不同研究。该蛋白的二级结构富含β折叠，表面具有糖类结合位点。 Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface. Application: Lectin from A rachis hypogaea (peanut) has been used: to determine the acrosomal status (presence or absence of acrosomal matrix corresponding to intact or acrosome-reacted spermatozoa) on viable sperm cells to probe cryosections of mediastinal lymph nodes (mLNs) with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs) to visualize the cone outer segments of pre-treated retinal sections General description Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit. Buffer：10 bicarbonate, 150 mM NaCl, pH 8.2, 0.1 mM Calcium chloride and 0.05% sodium azide

No numbering system information yet

No physical and chemical property information yet

No safety information yet

No production method and use information yet

No upstream information yet

No downstream information yet

No related product information yet