内切-β-半乳糖苷酶,E.C.:3.2.1.103,来源于 Bacteroides fragilis,内切β-半乳糖苷酶在大肠杆菌中重组表达的,是一种水解聚 n -乙酰乳胺结构中低聚糖(LNnT)内部β-半乳糖苷键的酶。对牛角膜硫酸角蛋白、牛奶低聚糖和糖脂类乳酸-n -新四糖基神经酰胺和乳酸-n -四糖基神经酰胺具有特异性。 使用方法 1.在反应体系中依次加入底物,50 mM 醋酸钠, pH 5.8,加入 5 mM CaCl2 混匀,加入适量内切-β-半乳糖苷酶。 用去离子水定容到反应体积。 2.将样品置于 37℃孵育 2-4 小时或者过夜。 【注】体系放大时候,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀,可以增加转化率。 注意事项 为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
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重组内切-β-半乳糖苷酶 (BfEndoβGal)
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 内-β-半乳糖苷酶 | ||
| English alias | keratan-sulfate endo-1,4-beta-galactosidase | endo-beta-galactosidase | β-Galactosidase | BfEndoβGal | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 生物活性 |
| PSA | - | logp | - |
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