Application This enzyme is useful for enzymatic determination of NH 3 , α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis. In vitro, various activity assays of this enzyme examine the conversion of α-ketoglutarate to L-glutamate, in the presence of excess ammonium ions (NH 4+ ) and NADPH. physical property Isoelectric point : 4.6 Michaelis constants : 1.1 X 10 -3 M (NH 3 ), 3.4 X 10 -4 M (α-Ketoglutarate) 1.2 X 10 -3 M (L-Glutamate), 1.4 X 10 -5 M (NADPH), 1.5 X 10 -5 M (NADP + ) Structure : 6 subunits (M.W.50,000) per mol of enzyme Inhibitors : Hg ++ , Cd ++ , p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine, 2,2′-dithiopyridine Optimum pH : 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG) Optimum temperature : 45 o C(α-KG−L-Glu) 45-55 o C (L-Glu→α-KG) pH stability : pH 6.0 - 8.5 (25 o C, 20hr) Thermal stability : below 50 o C (pH 7.4, 10min)
All
Chemical Reagents
Biomedicine
Organic raw materials
Inorganic chemical industry
intermediate
Agricultural chemicals
Auxiliaries and catalysts
Fragrances and Flavors
Dyes and Pigments
Daily chemical industry
L-谷氨酸脱氢酶（NADP型）
CAS number：unknown molecular formula：
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | L-Glutamate:NADP+ oxidoreductase (deaminating) | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | - |
| PSA | - | logp | - |
numbering system
No numbering system information yet
physicochemical properties
No physical and chemical property information yet
Safety information
No safety information yet
Production methods and uses
No production method and use information yet
Related
upstream information
No upstream information yet
downstream information
No downstream information yet
MSDS
Found 0 shareMSDS
