软骨素酶B(Chondroitinase B)来源于肝素黄杆菌(Flavobacterium heparinum),可降解硫酸皮肤素。此酶通过β消除机理作用于二糖重复单元之间的β1-4糖苷上并将其切断。降解反应的产物为寡糖,主要是不饱和二糖 。 用途 酶解 HPLC 法测定硫酸皮肤含量和二糖组成;肝素、硫酸乙酰肝素的精制;低分子硫酸皮肤素酶法制备。 推荐使用条件 最适pH:8.0 pH适用范围:5.5-9.5 最适温度:37 ℃ 温度适用范围:20~50 ℃ 使用缓冲液:20mmol/L Tris-HCl (pH:8.0),150 mmol/L NaCl, 5mmol/L CaCl2
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软骨素酶B
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 硫酸软骨素B酶 | 硫酸软骨素酶B | ||
| English alias | chondroitin B lyase | ChonB | chondroitinase B | cslB | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 生物活性 |
| PSA | - | logp | - |
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