β-内酰胺酶 Ⅳ

是否无菌：是 金属酶是采用重组基因的水溶性蛋白，经冻干后钴60辐照， 西林瓶的无菌包装。根据头孢菌素和培南类的化学结构而构建的重组基因蛋白。其基因片断的活性中心，可破坏头孢菌素和培南类抗生素的化学结构式，使开环/断链后失去该两类药物的抗菌性。 做抗生素的无菌检查时，应用手工注射器吸取无菌水注入药物的西林瓶中，摇匀，溶解，再吸出。加入至0.9%氯化钠的500ml溶液中，摇匀，不可用培养器上的针头溶解，输入，避免高浓度的溶液 通过滤膜，造成难以冲洗彻底。 这里，再次强调：做抗生素的无菌检查时，一定要手工注射器 注入无菌水溶解样品，再把溶解的样品转移至0.9%氯化钠的500ml溶液中摇匀，使得样品不会造成局部浓度过高，通过滤膜时难以冲洗彻底的情况。 无菌检验时，取金属酶冻干粉1瓶加入无菌水5ml溶解，摇匀，使成金属酶的溶液，取2ml酶的溶液放入1500ml的冲洗液摇匀。待 冲洗液冲洗培养器的滤膜完成后，泵尽。用手工注射器在三个培养器的呼吸口刺入，各加1ml的酶溶液，尽量让酶平铺在滤膜整个面， 静止10分钟，使高浓度的酶与培养器滤膜充分接触，以破坏（中和、灭活）滤膜上残留的头孢菌素和培南类药物，然后再泵入相应的 培养基，摇匀。阳性对照管加入1ml的大肠杆菌（100CFU/ml）。 在三个培养器加1ml的金属酶的溶液，可以去除培养器内部壁上和滤膜表面残留头孢菌素和培南类的少量药物抗菌性。 每天上午、下午各对阳性对照管轻轻摇匀一次。 客户可根据上述做方法学验证，也可根据实际操作情况做验证。 Is it sterile: Yes Metallozyme is a water-soluble protein using recombinant genes, lyophilized and irradiated with cobalt-60, and aseptic packaging of celine bottles. A recombinant gene protein constructed according to the chemical structure of cephalosporins and penan. The active center of its gene fragment can destroy the chemical structural formula of cephalosporins and penan antibiotics, so that the antibacterial properties of the two drugs are lost after ring opening/chain breaking. When doing sterility check of antibiotics, use a manual syringe to absorb sterile water and inject it into the drug vial, shake well, dissolve, and then suck it out. Add to 500ml solution of 0.9% sodium chloride, shake well, do not dissolve with the needle on the incubator, input, avoid high concentration of solution through the filter membrane, resulting in difficult to rinse thoroughly. Here, it is emphasized again: when doing sterile examination of antibiotics, it is necessary to inject sterile water into the manual syringe to dissolve the sample, and then transfer the dissolved sample to 500ml solution of 0.9% sodium chloride, so that the sample will not cause local concentration too high, difficult to wash thoroughly through the filter membrane. During sterility test, take 1 bottle of freeze-dried metal enzyme powder and add 5ml of sterile water to dissolve, shake well to make a metal enzyme solution, take 2ml of enzyme solution and put 1500ml of washing solution and shake well. After the rinse solution has washed the filter membrane of the incubator, the pump is exhausted. A manual syringe was used to Pierce the respiratory mouth of the three incubators, and 1ml of enzyme solution was added to each of them, and the enzymes were spread on the entire surface of the filter membrane as far as possible. Then, the high concentration of enzymes was fully in contact with the filter membrane of the incubator, so as to destroy (neutralize and inactivate) the residual cephalosporins and penem drugs on the filter membrane, and then pumped into the corresponding medium and shook well. Positive pairs were treated with 1ml of E. coli (100CFU/ml). Adding 1ml of the metal enzyme solution to the three incubators can remove the residual cephalosporins and penem on the inner wall of the incubators and the surface of the filter membrane. Shake the positive pair gently once a day in the morning and afternoon. Customers can do methodological verification according to the above, but also according to the actual operation of the verification.

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