Filipin III预制溶液

Filipin已在双染色操作中作为探针，用于检测聚丙烯酰胺凝胶中的脂蛋白并将其固定在硝酸纤维素膜上。它还广泛用于通过特定的荧光复合物对未酯化的胆固醇进行定位和定量。应用： Filipin III是一种多烯大环内酯类抗生素，是从S. filipinensis培养物中分离出来的主要成分。1 Filipin因其能够发出荧光并与胆固醇结合的能力而被广泛用作生物膜中甾醇定位的探针 2，以及在C型Niemann-Pick病的研究中，在临床上用作游离胆固醇的染色剂。 3 Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。 4 Filipin III被发现可在烟草细胞中引发信号反应，包括NADPH氧化酶依赖性产生。5。其抗真菌作用机制可能是通过与膜甾醇结合而改变膜通透性和相关功能。Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。 Filipin III is a polyene macrolide antibiotic and the major component isolated from cultures of S. filipinensis. 1 Due to its ability to fluoresce and bind to cholesterol, Filipin has been widely used as a probe for sterol location in biological membranes 2 and has been used clinically as a stain for free cholesterol in the study of Type C Niemann-Pick disease. 3 Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. 4 Filipin III was found to trigger signaling responses in tobacco cells, including a NADPH oxidase-dependent production. 5 . The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. Application: Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.

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