p-Nitrophenyl Phosphate

ELISA应用： 对硝基苯基磷酸酯以50X浓缩液（0.19 mol/l）的形式提供。与对-NPP 底物缓冲液（货号 487664）一起使用。对硝基苯基磷酸酯是基于碱性磷酸酶的 ELISA 测定中最常见的底物。磷酸酯水解释放出对硝基苯酚，它是一种可溶的黄色色原体，可在 405 nm（ε= 1.83 x 104M-1cm-1）以分光光度法测定。由于反应是线性的，因此可以进行动力学分析。终点的确定也可以通过用强碱终止反应并测量 405 nm 处的吸光度来进行。这种p-NPP 制剂是一种在专有溶剂中的稳定、便捷的液体浓缩物。基于碱性磷酸酶的ELISA分析的出色底物。产生黄色可溶成品，可在405 nm至410 nm处读取。可以进行动力学和终点测定。以即用型产品提供。可单独购买的即用型稀释剂（货号487664）。这种对-硝基苯基磷酸酯经过验证可用于 ELISA。 p -Nitrophenyl phosphate is the most common substrate for alkaline phosphatase-based ELISA assays. Hydrolysis of the phosphate ester liberates p -nitrophenol, a soluble, yellow chromogen that can be measured spectrophotometrically at 405 nm (ε = 1.83 x 10 4 M -1 cm -1 ). As the reaction is linear, kinetic assays can be performed. Endpoint determinations can also be performed by stopping the reaction with strong base and measuring the absorbance at 405 nm. This p -NPP preparation is a stable, convenient, liquid concentrate in a proprietary solvent. p -Nitrophenyl phosphate supplied as a 50X concentrate (0.19 mol/l). For use with p -NPP Substrate Buffer, Cat. No. 487664. Excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. Supplied as ready-to-use. A ready-to-use diluent is available separately (Cat. No. 487664 ). This p -Nitrophenyl Phosphate is validated for use in ELISA. Application: ELISA

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