compound introduction
ANB-NOS和磺基-SANBAH是异双官能交联剂，含有胺反应性的N-羟基琥珀酰亚胺酯和光活化的硝基苯叠氮化物。NHS酯在pH 7-9缓冲液中与伯氨基（-NH2）有效反应，形成稳定的酰胺键。当暴露在紫外光下时，硝基苯叠氮化合物形成氮烯基团，该基团可以引发双键的加成反应，插入碳氢和氮氢位点，或随后与亲核体（例如伯胺）发生扩环反应。 ANB-NOS and Sulfo-SANPAH are heterobifunctional crosslinkers that contain an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. When exposed to UV light nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).
