Membran Substrate

一、产品属性 保质期: 36个月 标记酶: 碱性磷酸酶(AP) 反应时间: 较短(20-25°C下，可达20分钟) 批间差: 肉眼不可见 沸点：100℃ 酸碱度范围: 9.5-9.9 密度（20℃）：1.007 g/cm³ 溶解性：易溶于水 外观：黄色液体 气味：无味 Light sensitive 对光线敏感 Heat sensitive 对热敏感 二、保存及运输条件： 1. 保持容器密闭 2. 储存温度2 °C - 8 °C，避光 3. 不与氧化剂一起储存 4. 在环境温度不超过 30 °C，运输时间应少于一周。 5. 在储存过程中，自发反应可能会产生细小的暗沉淀。这不会影响性能。 6.过期后，溶液仍可使用，但是产品活性可能会降低； 7. 从损坏的瓶子中污染或泄漏的基质溶液不可再使用，须销毁。 三、有效成分及生化机理 Tris -缓冲液(pH 9.7)缓冲体系含有以下有效成分: 5‑Brom‑4‑chlor‑3‑indolylphosphate (0.5 mM)，缩写为 BCIP Nitrotetrazoliumblue (1.0 mM)，缩写为 NBT 碱性磷酸酶水解BCIP的磷酸残基。 剩余吲哚基残基为还原剂。在与周围空气中的氧气接触时，它二聚形成一种浅蓝色的复合体。这种反应是不需要的。在黄色NBT的存在下，它被还原并在环结构打开后形成深紫色甲臜染料。 四、生物安全信息 根据法规(EC) No 1272/2008，本产品不属于危险类别； 五、主要优点 1. 由于高活性而快速形成沉淀 2. 非常低的背景有助于形成高度的对比 3. 暗紫色沉淀 4. 反应停止后无明显褪色 六、使用说明 关于分装的建议 •在没有灰尘和黑暗的房间工作。 •注意溶液不要接触到仪器金属部件(导致催化) ，硅管的封闭系统是有利的。 •仔细清洁所有的仪器和容器。 •不要用裸露的手触摸与溶液接触的仪器部分。戴无粉手套。 •立即关闭瓶子，尽量减少光线和灰尘的影响。 •使用由高密度聚乙烯或聚丙烯制成的不透光的瓶子。 七、使用的一般说明 只有熟悉免疫学方法基础的合格实验室工作人员才能使用该产品。 本产品适用于具有视觉解释的印迹反应体系。在规定的条件下，可进行密度测量。 根据系统的不同，在与结合物孵育后，在转移到底物溶液中之前充分清洗测试条。条带通过在轨道振荡器上移动而显影，直到出现明显的颜色（10‑20 分钟）。 大多数商业膜都可以清洗和干燥。在干燥的条带上可以看到最佳的颜色对比。干燥的条状可用于记录。 （注意：某些胶体会破坏有色沉淀物。） 1、Product attribute Shelf life: 36 months Marker enzyme: alkaline phosphatase (AP) Reaction time：short (up to 20 minutes) at 20-25°C Lot-to-lot variation：not visible by naked eye Boiling point : 100℃ pH-Value (at 20 °C): 9.5-9.9 Density (20℃) : 1.007 g/cm³ Water solubility: easily soluble Appearance: yellow liquid Odour: odourless Light sensitive Heat sensitive 2、Requirements for storage rooms and vessels 1.Keep container tightly closed. 2.Keep cool. protected from light 3.Do not store together with: Oxidizing agent. 4. It is possible to transport the solution at ambient temperature, unless it does not exceed 30 °C. The transport should take less than one week. 5.During storage a fine dark precipitation may occur by spontaneous reaction. This does not affect the performance. 6.The solution still works even beyond the expiry date, but a lower activity has to be taken in account. 7. Contaminated or leaked out substrate solution from damaged bottles should not be used anymore and has to be destroyed. 3、 Effective Components and Principle of Function A Tris – buffer (pH 9.7) system contains the following effective components: • 5-Brom-4-chlor-3-indolylphosphate (0.5 mM), abbreviated BCIP • Nitrotetrazoliumblue (1.0 mM), abbreviated NBT Alkaline Phosphatase hydrolyses the phosphate residue from BCIP. The remaining Indoxyl residue is a reducing agent. In contact with oxygen from surrounding air it dimerises to a light-blue coloured complex. This reaction is not desired. In presence of the yellow NBT，this is reduced and forms a dark purple formazan dye after opening of the ring structure. 4、Biosafety information This mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008； 5、Advantage 1. Rapid precipitate formation due to high activity 2. Very low background contributes to a high contrast 3. Dark purple precipitates 4. No significant fading after reaction stop 6、Instruction for usage For bottling consider the following instructions: • Work in a dust free and darkened room. • Pay attention that the solution has no contact with metal parts (leading to catalysis) of your instruments. Closed systems of silicon tubes are favoured. • Clean all instruments and vessels very carefully. • Never touch parts of the instruments that are in contact with the solution with the naked hand. Wear powder free gloves. • Close the bottles immediately to minimize the influence of light and dust. • Use bottles that are not permeable to light, made from HDPE or PP. 7、 General Instructions for the Use in Blotting Systems Only qualified laboratory staff, who are familiar with the basics of immunological methods, are allowed to use this solution. The substrate solution is intended for the use in blotting systems with visual interpretation. Under defined conditions a densitometric measurement is possible. Depending on the system it is sufficient to wash the test strips after incubation with conjugate prior to transfer into the substrate solution. The strips are developed by movement on an orbital shaker until a distinct colour appears (10-20 minutes). Most commercial membranes are allowed to be washed and dried. The best colour contrast is visible on dry strips. The dried strips may be stored for documentary purposes. (Pay attention: some kinds of glue destroy the coloured precipitate.)

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