Stabilizers for solid phase

一、产品属性 酸碱度范围: 7.2 - 7.6 防腐剂: 叠氮化物 沸点：100℃ 外观：无色液体 密度（20℃）：1.0251 g/cm³ 二、保存及运输条件： 1. 保持容器密闭 2. 储存温度2 °C - 8 °C. 3. 只保留/储存在原始容器中 4. 切勿将溢出的液体放入原容器中重复使用 5. 应远离食品和饲料 6. 在环境温度不超过 30 °C，运输时间应少于一周。 三、有效成分及生化机理 本产品是一种含有牛血清白蛋白（封闭剂）、 蛋白质稳定物质和叠氮化物（防腐剂）的磷酸盐缓冲溶液。 在涂布微量滴定板或其他带有用于免疫反应的活性蛋白的塑料表面的时候，表面的某些部分可能不会被涂覆。为了避免反应体系发生非特异反应，未被涂覆的部分将被本产品中含有的大量的白蛋白覆盖；此外，本产品中的特殊添加剂可以稳定蛋白质结构，从而保持已涂布微量滴定板在储存过程中蛋白质的功能。 四、生物安全信息 叠 氮化物对水生物有危险，可能与重金属离子形成爆炸物。 根据法规(EC) No 1272/2008，本产品不属于危险类别； 根据法规(EC) No 1907/2006 (REACH)， 本产品没有有害物质； 根据(EC) No 1272/2008，本产品不含有任何对健康或环境造成危害或指定职业接触限度的物质 五、主要优点 1.用于稳定固相吸附蛋白质的后涂层溶液； 2.有效减少非特异性结合 六、使用说明 1.任何填充其他容器或拆分的操作都必须在无菌条件下进行； 2.冷冻溶液解冻后必须充分混合后使用； 3.不应使用显示浑浊的溶液（因为这可能是微生物污染的迹象）。 4.只有熟悉免疫测试生产的合格人员才能处理。 5.取决于包被后的反应性蛋白吸出物，用 PBS 或封闭溶液洗涤一次。 6. 微量滴定板的孔应填充 200 至 300 µl 未稀释的本产品，并在室温下孵育一小时。封闭溶液的体积必须大于涂层溶液的体积以及预期的测试体积。 7.吸出底物以完成封闭过程。可选用去离子水清洗一次。在室温或 37°C 的无尘处干燥表面并密封，放入添加干燥剂（硅胶）的袋中。 1、Product attribute pH Range: 7.2 - 7.6 Preservative: Azide Boiling point : 100℃ Appearance: Colorless liquid Density (20℃) : 1.0251 g/cm³ 2、Requirements for storage rooms and vessels 1.Keep container tightly closed. 2.Keep cool. 3.Keep/Store only in original container. 4.Never return spills in original containers for reuse. 5. Keep away from: Food and feeding stuffs 6. It is possible to transport the solution at ambient temperature, unless it does not exceed 30 °C. The transport should take less than one week. 3、Effective Components and Principle of Function It is a phosphate buffered solution containing bovine serum albumin as blocking reagent, protein stabilizing substances and azide as preservative. During the coating of micro titration plates or other plastic surfaces with reactive proteins for immunological reactions, parts of the surfaces may stay uncoated. Due to avoid non specific reactions in the test system, these parts will be blocked with albumin, which is contained in this product in high excess. Special additives of this product additionally stabilize the protein structure and therewith preserve protein functionality during storage of the coated micro titration plate or other coated plastic surfaces. 4、 Biosafety information Azide is dangerous for water organisms and may form explosives in connection with heavy metal ions. This mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008； Hazardous components：none (according to Regulation (EC) No 1907/2006 (REACH))； This mixture does not contain any substances which either present a health or environmental hazard according to Regulation (EC) No. 1272/2008 or have an occupational exposure limit assigned 5、 Advantage 1. Postcoating solution for stabilizing solid phase adsorbed proteins； 2. Effective reduction of nonspecific binding 6、 Instruction for usage 1. Any handling to fill in other vessels or splitting has to be done under sterile conditions. 2. Frozen solutions have to be mixed thoroughly after thawing and can be used without restriction afterwards. 3.Solutions showing turbidity should not be used. Since this might be a sign of microbial contamination. 4. Only qualified staff, who are familiar with the production of immunological tests, is permitted to handle this product. 5. Depending from the reactive protein aspirate after coating or wash once with PBS or blocking solution. 6. Wells of micro titration plates should be filled with 200 to 300 µl undiluted this product and incubated for one hour at room temperature. The volume of the blocking solution has to be higher than the volume of the coating solution as well as the intended test volume. 7. Aspirate this product to finish the blocking process. Optional wash once with deionized water. Dry the surfaces at a dust free place at room temperature or 37°C and seal airtight, in a pouch with addition of desiccant (silicagel).

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