Heterophilic Blocking Reagent

纯化抗体适度聚合为大分子复合物，构成重复结合表位，更利于干扰物质的结合，同时，发挥大分子的位阻效应，抗干扰能力大大加强，对各类干扰尤其是多价干扰有显著消除或降低作用；适用于所有免疫检测平台LF、CLIA、EIA、TIA等。纯度大于95%，浓度10mg/ml（0.01M PBS，pH7.4），0.2μm过滤，不添加防腐剂。 聚合阻断剂专门针对免疫检测中发生的异嗜性抗体和类风湿因子干扰，适用于免疫检测体系，能有效消除免疫干扰，提高检测的可靠性，减少假阳性和假阴性的发生率。 1. 阻断剂使用浓度范围为 30µg-300µg/ml。最终工作浓度应通过梯度实验确定，建议首次使用浓度从较高浓度开始，确定有效果后再梯度稀释至较低浓度，选择更合适经济的使用浓度。 2. 免疫侧向层析法试剂建议加至标记物垫或样品垫处理液中；化学发光试剂或酶联免疫试剂建议加至磁珠液或酶标液中，也可添加至样品液中；免疫比浊产品建议加至 R1 工作液中。 3.建议选择多种阻断剂进行试验，寻找合适的单一阻断剂产品或阻断剂组合产品使用。 Purified antibodies are moderately polymerized into macromolecular complex, forming repeated binding epitopes, which is more conducive to the binding of interfering substances. At the same time, it exerts the steric hinsteric effect of macromolecules, greatly enhancing the anti-interference ability, and significantly eliminating or reducing all kinds of interference, especially multivalent interference. Applicable to all immunoassay platforms LF,CLIA,EIA,TIA and so on. Purity greater than 95%, concentration of 10mg/ml (0.01M PBS, pH7.4), 0.2μm filtration, no preservatives added. Polymerization blockers are specifically targeted at the interference of heterophil antibodies and rheumatoid factors in immune detection, and are suitable for immune detection system. They can effectively eliminate immune interference, improve the reliability of detection, and reduce the incidence of false positive and false negative. 1. The concentration range of the blocker should be 30µg-300µg/ml. The final working concentration should be determined by gradient experiment, which is recommended for the first time The use of concentration from a higher concentration, to determine the effect of the gradient dilution to a lower concentration, choose a more appropriate economic With concentration. 2. Immunolateral chromatography reagents are recommended to be added to the marker pad or sample pad treatment solution; Chemiluminescent reagent or enzyme linked immunoassay The agent is recommended to be added to the magnetic bead solution or the enzyme label solution, or to the sample solution; Immunoturbidimetric products are recommended to be added to R1 for work In the liquid. 3. It is suggested to select a variety of blockers for testing, and find a suitable single blocker product or combination of blockers for use.

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