类器官回收液是一款不含酶的即用型溶液,可用于从ECM基质胶中回收完整类器官,而对类器官的形态或结构产生破坏。本产品回收获得的类器官可用于铺板、传代、冻存、成像、病原体感染,RNA以及蛋白提取等。 使用说明: 1、为避免枪头粘附性损耗,可将1mL移液枪头吹打抗粘附液进行润洗枪头。 2、刮取含类器官的基质胶滴或基质胶层,转移至1.5mL或15mL离心管中,加入10倍体积的类器官回收液。 3、冰上孵育30min。 4、使用水平转子离心机,50g离心3min,小心吸弃上清。 5、用基础培养基或PBS重悬细胞,50g离心3min,小心吸弃上清。 6、将上述获得的类器官用于后续实验。
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Organoid Recovery Solution
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | Cell recovery solution for Organoid | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无菌过滤 |
| PSA | - | logp | - |
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