RPMI 1640培养基以研发地点罗斯韦尔公园纪念研究所(Roswell Park Memorial Institute. RPMI)命名,1640为培养基代号。它是McCoy's 5A培养基的改进型,使用碳酸氢盐缓冲系统。RPMI 1640培养基最初开发用于人白血病细胞的悬浮或单层培养,后来被发现也适用于多种哺乳动物细胞,包括HeLa、Jurkat、MCF-7、PC-12、PBMC、星形胶质细胞和癌细胞。 RPMI 1640培养基与其它培养基的区别在于含有还原型谷胱甘肽和高浓度的维生素。 RPMI 1640培养基含有EMEM和DMEM中没有的生物素、维生素B12和对氨基苯甲酸,以及高浓度的氯化胆碱和肌醇。 RPMI 1640培养基(ATCC改良型)是在常规RPMI 1640基础上,额外添加了丙酮酸钠和HEPES,增加了葡萄糖的含量,降低了碳酸氢钠的含量。 本产品含有:D-葡萄糖(4.5g/L)、酚红、L-谷氨酰胺、丙酮酸钠、HEPES。
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RPMI 1640 Medium (ATCC modification)
CAS number:unknown molecular formula:
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| Chinese alias | RPMI1640培养基 (ATCC改良型) | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无菌过滤 |
| PSA | - | logp | - |
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